Mirna Array

The genetics of human complex traits, Mendelian disorders, and populations are also application arrays that can be advanced with Affymetrix microarray analysis, such as through population-optimized and application-optimized genotyping to enable human genetic research workflows.

Mirna array. MiRNA PCR Arrays ONLY. To select multiple options in Windows, hold down the control (ctrl) key;. Diagram of MicroRNA biogenesis, processing and function.

TaqMan Advanced miRNA array cards. MiRNA probes are each spotted three times. If you don’t see the array you are interested in, you can find out about the full catalog of arrays that are available from Affymetrix at their website and then talk with us to discuss pricing.

Leukemia (09) 23, 1790-1800;. PhD-trained application specialists are available to provide technical support for the arrays, and each array can also be modified to suit unique experimental needs. NuRNA™ tRNA Modification Enzymes PCR Array (H) NuRNA™ Small RNA Biogenesis Proteins PCR Array (H).

Custom miRNA assay for Human, Mouse, or Rat made from -50 miRNAs taken from the list of miRNAs found in the Human, Mouse, or Rat assay. Many species are available. GeneChip™ miRNA Arrays are powerful tools for studying the role of small non-coding RNA (miRNA, snoRNA, and scaRNA) in complex diseases, such as cancer.

MiScript miRNA PCR Array — Cutting-edge miScript miRNA PCR Arrays consist of mature miRNA-specific forward primers (miScript Primer Assays) arrayed in biologically relevant pathway- or disease-focused panels or miRNome panels. (B) Photogenerated charge transfer mechanism of cascaded multiple photosensitive structures (VB, valence band;. TaqMan array cards are the ideal solution for profiling large numbers of miRNAs and samples with limited material (each reaction requires only 2 µL of sample).

A proprietary probe design and unique labeling method provide optimal sensitivity and specificity. NrStar™ tRF&tiRNA PCR Array (H/M) snoRNA PCR Array. Sensitive and accurate detection of targets in different sample materials, including complex sample types or those with low miRNA expression levels (e.g., liquid biopsies) is critical for your miRNA qPCR or miRNA quantification.

If you have your own miRNA sequences or would like custom sequences built into arrays, our custom array is the way to go. Citation needed Such experiments can generate very large amounts of data, allowing. This array design is a powerful tool for studying the role of small non-coding RNAs and their involvement in a broad spectrum of developmental and physiological mechanisms.

Agilent miRNA microarrays are a unique platform able to analyze only mature miRNA as highlighted in the 14 miRQC* platform benchmark study. With clones for miRNA precursors, inhibitors, and 3' UTR targets, to primers and kits for miRNA qPCR expression profiling, GeneCopoeia's miRNA Complete Solutions furnish you with a wealth of tools to help you every step of the way in your miRNA analysis workflow. Samples are prepared using Flashtag labeling kits.

423 miRNAs, 4 mRNAs, and 25 internal reference controls. The qPCR system you use needs to give accurate, highly specific results. The miProfile human miRNome miRNA qPCR arrays are a set of nineteen 96-well plates covering 1,700 of best characterized and annotated miRNAs based on miRBase V18.

We identified a three-miRNA signature, hsa-miR-140-3p, hsa-miR-671-3p, and hsa-miR-33b-3p, in the serum of OA patients, predicted to regulate metabolic processes, which could serve as …. The system is based on the LNA™-technology, which offers unique advantages in the specific and sensitive detection of short RNA sequences. Available for human, mouse, and rat.

Affymetrix® miRNA 3.1 Array Strip (pdf, 84 KB) Affymetrix® miRNA 3.1 Array Strip (pdf, 7 KB) -Japanese Version. Human (HmiOA), Mouse and Rat (MRmiOA), and Model Plant species (MPmiOA). While they are the least quantitative of the three miRNA assay methods, conventional DNA oligonucleotide arrays are a relatively inexpensive way to measure hundreds of targets at once.

MiRNA 4.0 and 4.1 Array (pdf, 74 KB) miRNA 4.0 and 4.1 datasheet (pdf, 515KB) - Japanese version. An analysis of the results from two qPCR-based platforms (miRCURY Ready-to-Use PCR, Exiqon, and TaqMan Human MicroRNA Array v3.0, Life Technologies) with an array-based system (GeneChip miRNA 2.0 Array, Affymetrix) found that the array-based platform has a lower sensitivity and that the miRCURY system has a better sensitivity than the TaqMan. In Macs, hold down the command key.

Learn about the miRNA complete labeling and hybridization kit. Independent experiments demonstrated that Agilent miRNA microarrays exhibited the most consistent performance in miRNA detection, superior to qPCR, sequencing, and other microarray platforms. The array provides comprehensive miRNA coverage (Sanger miRNA database V content and additional human small nuclear RNAs (snoRNAs and scaRNAs)) with multiple organisms (Human, mouse, rat, canine, and monkey) on a single array.

The price includes arrays, reagents, processing, and basic gene-level data analysis. We have standard arrays for all mature miRNAs of all species available in the latest version of the miRBase database (Release 21, July 14). The reference miRNA will appear in a new window to the right.

OneArray® microarrays have been manufactured for 10. Chromosome 21/22 2.0R Array;. The miProfile™ exosome arrays allow researchers to profile the differential expression of miRNAs in exosomes from different samples (urine, blood, or milk) and various cancers to gain understanding of the role of miRNA in the intercommunication occurring between different cells.

This enhanced version of its best-in-class microRNA interrogation solution will support the research community’s pursuit of personalized medicine by accelerating the identification of biomarkers and gene expression signatures associated with disease. Our content is designed with a clinically actionable biology-driven approach from miRbase V21. Relatively inexpensive way to measure hundreds of targets at once;.

The miProfile miRNA PCR Arrays are designed for profiling the expression of pre-defined or customized sets of miRNAs in various tissues or cells. QuantiGene miRNA Singleplex Assay QuantiGene™ miRNA Singleplex Assays are 96-well, hybridization-based assays that utilize a branched DNA technology for signal amplification for the direct quantitation of target miRNAs. NrStar™ tRNA PCR Array (H/M) tRF&tiRNA PCR Array.

Search for pre-designed 96 well plates › 384-well microfluidic TaqMan Array card:. The Cancer MicroRNA qPCR Array with QuantiMir™ enables the discovery of new MicroRNA signatures using 95 different MicroRNAs known to be involved in apoptosis, cell fate, development, and cancer from a diverse set of RNA samples. A DNA microarray (also commonly known as DNA chip or biochip) is a collection of microscopic DNA spots attached to a solid surface.Scientists use DNA microarrays to measure the expression levels of large numbers of genes simultaneously or to genotype multiple regions of a genome.

The GeneChip miRNA Array is a powerful tool for studying the role of small non-coding RNAs and their involvement in a broad spectrum of developmental and physiological mechanisms. MiRNA biomarker discovery for serum and plasma with simultaneous analysis of mRNA and miRNA from the same sample. When the sequences are perfectly matched, the oligos are aligned with the miRNA and the joint can be ligated by DNA ligase (figure 1).

Genechip miRNA The GeneChip miRNA 4.0 array contains content to screen for all mature miRs from miRBase Release. Built on the success of the first-generation miRNA Array design, turnkey assay, and. Human Tiling 1.0R Array Set;.

MiRNA arrays Conventional DNA oligonucleotide arrays are typically chosen for larger studies covering multiple miRNA targets. MiRNA Arrays - Support Materials For more information, please see the product page. Ordering information Part number Description Details GeneChip® miRNA 4.0 Array Contains 2 arrays GeneChip® miRNA 4.0 Array Contains 6 arrays GeneChip® miRNA 4.0 Array Contains 30 arrays GeneChip® miRNA 4.0 Array and Flashtag™ Bundle Sufficient for 10 samples GeneChip® miRNA 4.0 Array and Flashtag™ Bundle Sufficient for 30 samples.

Flash™ Biotin HSR RNA Labeling Kit (pdf, 217 KB) miRNA Arrays (pdf, 1.85 MB) Library Files. A single nucleotide difference among miRNAs will block either the hybridization or. The kit combines a unique miRNA direct labeling method with our innovative microarray probe design for miRNA array labeling and hybridization of mature miRNAs.

This array can simultaneously profile more than 1,500 mature miRNAs and their corresponding precursors from 474 human and 373 mouse miRNA genes. Thermo Fisher Microarray Instruments, Software, and Services. The resulting differential expressions of profiled miRNAs help researchers to identify those miRNAs that are of biological significance and importance relevant to their research.

5S rRNA, tRNAs, U6). LC Sciences provides a microRNA (miRNA) expression profiling service using microarrays based on our in-house developed µParaflo® technology platform. Published online 21 May 09″ “Neuropeptides coordinate complex social behaviors important to both basic and applied science.

NrStar™ snoRNA PCR Array (H/M) microRNA PCR Array. Custom GenoExplorer™ miRNA microarrays help you build customized miRNA arrays, such as the known miRNA sequences and genome-wide predicted miRNA sequences. Click the “Add button”.

Schematic illustration of (A) ratiometric PEC paper analytical device with dual-photocathode array for miRNA-141 detection, the color scheme of test pad is the same as in Scheme 1A. Human Tiling 2.0R Array Set;. OneArray® miRNA microarrays are available for:.

Learn about SurePrint human miRNA microarrays. MiRNA 2.0 Array (pdf, 1.53 MB) miRNA 3.0 Array (pdf, 4.8MB) - Japanese Version. Drosophila Tiling 2.0R Array;.

Predesigned TaqMan Advanced miRNA array cards are now available, as well as custom TaqMan array cards containing TaqMan Advanced miRNA assays or the classic chemistry TaqMan miRNA assays that are ordered using our configurator tool. The arrays are pathway-focused panels of laboratory-verified qPCR assays, with integrated, patented controls to ensure a successful experiment every time. Positive and negative control probes (eg.

Mature technology with well-established protocols and purpose-built analysis tools available. A serum miRNA signature was established for the first time using high density resolution miR-arrays in OA patients. A minimum of 130 nanograms of total RNA is recommended.

Microarray analysis techniques are used in interpreting the data generated from experiments on DNA (Gene chip analysis), RNA, and protein microarrays, which allow researchers to investigate the expression state of a large number of genes - in many cases, an organism's entire genome - in a single experiment. To keep pace with the discovery of new and novel miRNA, we are pleased to offer the GeneChip™ miRNA 4.0 Array to our growing catalog of miRNA arrays. These human microRNA microarrays offer comprehensive coverage with the latest content.

The miRNA array consists of 4,104 probes printed in duplicate. Each DNA spot contains picomoles (10 −12 moles) of a specific DNA sequence, known as probes (or reporters or oligos). Scroll through the custom miRNA list and select the desired reference miRNA by clicking it.

Drosophila Tiling 1.0R Array;. Launched the GeneChip miRNA 2.0 Array. The GeneChip® miRNA Array is a powerful and cost effective tool for studying the role of microRNAs (miRNAs).

The miProfile miRNA PCR Arrays are designed for profiling the expressions of pre-defined or customized sets of miRNAs in various tissues or cells. The miRCURY LNA™ microRNA array system is a complete system for global microRNA expression profiling. Human Promoter 1.0R Array;.

A proprietary probe design and unique labeling method ensures optimal sensitivity and specificity. The resulting differential expression of profiled miRNAs help researchers to identify those miRNAs that are of biological significance and importance relevant to their research. It is a two-dimensional array on a solid substrate—usually a glass slide or silicon thin-film cell—that assays (tests) large amounts of biological material using high-throughput screening miniaturized, multiplexed and parallel processing and detection methods.

The updated miRNA sequences with mature and most precursor probes all on one array. Rat v1.5 miRNA Assay:. MiScript miRNA PCR Arrays are available in 96-well, 384-well, and Rotor-Disc 100 formats (see figures " Pathway-Focused or miRNome miScript miRNA PCR Array layout for plate formats A, C, D, F, M ", " Pathway-Focused miScript miRNA PCR Array layout for plate formats E, G ", " miRNome miScript miRNA PCR Array and miScript miRNA HC PCR Array layout for plate formats E, G ", and " miScript miRNA PCR Array layout for Rotor-Disc format R ").

Expression Microarray Reagent Guide (pdf, 244 KB) Data Sheets. À la carte miRNA:. In our array assay, each miRNA molecule is targeted by two oligos that hybridize with the target miRNA to form a RNA/DNA duplex.

Agilent miRNA microarrays exhibited the most consistent performance in miRNA detection. TaqMan Advanced miRNA 96 well array plates Comprehensive collection of pre-plated, pre-designed panels available in both fast and standard 96 well formats. NrStar™ Canonical Conserved miRNA PCR Array (H) mRNA PCR Array.

Elegans Tiling 1.0R Array;. Our catalog SurePrint Human, Mouse & Rat miRNA Microarrays provide comprehensive coverage of miRNAs with the most up-to-date content. Affymetrix® miRNA Array Plate is a powerful tool for studying the role of small non-coding RNA (miRNA, snoRNA, and scaRNA) and their importance in cancer and other diseases.

Arrays are typically chosen for larger studies covering multiple miRNA targets. Arabidopsis Tiling 1.0R Array;.