Exo Sap
It is used to remove excess primers and dNTPs from a PCR mixture with no interference in downstream applications.
Exo sap. The ExoSAP-IT one-step solution provides a simple way to enzymatically purify a PCR reaction. The exonuclease I removes leftover primers, while the Shrimp Alkaline Phosphatase removes any remaining dNTPs. The first incubation digests excess primer and dephosphorylates nucleotides.
Our sequencing Exo-SAP-IT PCR product cleanup reagent provides fast, easy, and convenient solutions for your PCR cleanup needs. The second, high-temperature incubation inactivates the enzymes. ExoSAP-IT Express reagent consists of two hydrolytic enzymes, a modified Exonuclease I and Shrimp Alkaline Phosphatase (SAP), in a specially formulated buffer.
The reaction setup is complete with one pipetting step, which is followed by two incubations. ExoSAP-IT single-step PCR cleanup utilizes two hydrolytic enzymes, Exonuclease I and Shrimp Alkaline Phosphatase (SAP), together in a specially formulated buffer, to remove unwanted dNTPs and primers from PCR products. EXO-Skin Sap Flow Sensor Patent US B1 After more than 25 years of experience and a worldwide experience with sap flow sensor manufacturing and design, the Dynamax team brings you the latest innovations and sap flow sensor construction advancements.
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