Cleaved Caspase 3 Antibody

Caspase 3 is cleaved at Asp28/Ser29 and Asp175/Ser176. This antibody does not recognize full length caspase-3 or other cleaved caspases. Cleaved Caspase-3 (Asp175) (5A1) Rabbit mAb detects endogenous levels of the large fragment (17/19 kDa) of activated caspase-3 resulting from cleavage adjacent to Asp175.
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Secondary - Goat Anti-Rabbit IgG H&L (HRP) (ab5718) IHC-P, WB, ELISA, IP.

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Cleaved caspase 3 antibody. Caspase 3 Monoclonal Antibody (3CSP03 (4.1.18)). Cleaved Caspase-3 (Asp175) Antibody detects endogenous levels of the large fragment (17/19 kDa) of activated caspase-3 resulting from cleavage adjacent to Asp175. Immunohistochemistical detection (on formaldehyde/PFA-fixed paraffin-embedded sections) of cleaved Caspase-3 antibody (ab2302) on Quail Tissue sections (Quail E6/7 developing DRGs Saggital section).

Caspase-3 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Rabbit Anti-Human/Mouse Cleaved Caspase-3 (Asp175) Monoclonal Antibody (Catalog # MAB5) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Raised against amino acids 1-277 representing the full length precursor form of caspase-3 of human origin. This antibody detects non-specific caspase substrates by western blot.

Caspases 6, 7 and 9), as well as relevant targets in the cells (e.g. This antibody does not recognize full length caspase-3 or other cleaved caspases. Protein - Recombinant human Cleaved Caspase-3 protein (Active) (ab) Functional Studies.

Anti-caspase-3 Antibody (E-8) is a mouse monoclonal IgG 2a ( kappa light chain) caspase-3 antibody provided at 0 µg/ml. Caspase-3 Antibody detects endogenous levels of full length caspase-3 (35 kDa) and the large fragment of caspase-3 resulting from cleavage (17 kDa). The active Caspase 3 proteolytically cleaves and activates other caspases (e.g.

Caspase-3 Antibody (E-8) is recommended for detection of caspase-3 and full length procaspase-3 of human origin by WB, IP, IF, IHC (P), FCM and ELISA. 1% BSA for 10 mins RT. Ab recognizes a cleaved form of Caspase 3 (~17 kDa) after apoptosis has been induced in wildtype cells and not Caspase 3 knockout cells.

View more associated products. Primary - Rabbit Anti-Cleaved Caspase-3 antibody EPR (ab) WB. Primary Antibody ab2302 incubated at 1/50 for at RT.

Variations in levels of Caspase 3 have been reported in cells of short-lived nature and those with a longer life cycle. Some customers have used this antibody successfully in IHC-P however our latest tests were unsuccessful and therefore we can no longer guarantee this application.

Figure 3 Morphological And Cytochemical Determination Of Cell Death By Apoptosis Springerlink

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